hi guys,
if the problem wasn't solved by tweaking the .htaccess file then you might want to check the permissions on the files that you uploaded to your site. compare them with the permissions on the unpacked download that you got from the roundcube site. sounds like roundcube install is fine and your server is probably also fine. this is something with the app itself having some difficulty running.
On my first attempt at installing roundcube i got exactly the same error & message. I discovered it was an issue with my ftp-client changing all the permissions, chmod-ing them to 644. i think this happened because i decided i would change the name of the directory i put the roundcube files into :)
if that doesn't solve you problem, what does you error log file say?
HTH,
lucy